Capillary Zone Electrophoresis for the Diagnosis of Congenital Hemoglobinopathies

Capillary zone electrophoresis for the diagnosis of congenital hemoglobinopathies.

by addition of calculated amounts of 200 g/L glucose. Serum glucose concentrations attained by this procedure were controlled by measurement with the Beckman Glucose Analyzer. The sensitivity to glucose (calculated for the range 5–10 mmol/L) in phosphate buffer (pH 7.4) was 10.2 Ϯ 3.3 nA ⅐ L ⅐ mmol Ϫ1 (mean of slopes calculated from each experiment Ϯ SE; Fig. 1A).The sensitivity to glucose afte...

Diagnosis of congenital disorders of glycosylation by capillary zone electrophoresis of serum transferrin.

BACKGROUND Congenital disorders of glycosylation (CDG) are usually diagnosed by isoelectric focusing (IEF) of serum transferrin (Tf). The aim of this study was to evaluate capillary zone electrophoresis (CZE) as a diagnostic alternative to IEF. METHODS We performed 792 CZE analyses of Tf, using the CEofix(TM)-CDT (carbohydrate-deficient transferrin) assay. Peak identification was based on rel...

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Multicenter validation of fully automated capillary electrophoresis method for diagnosis of thalassemias and hemoglobinopathies in Thailand.

Thalassemias and hemoglobinopathies are highly prevalent in Thailand and other Southeast Asian countries. Accurate and precise separation of hemoglobin types, together with reliable quantitation, are essential for differential diagnosis of these diseases. Presented in this study is a multicenter validation of a fully automated capillary electrophoresis (CE) method for hemoglobin separation and ...

Evaluation of Acetonitrile Deproteinisation of the Serum Samples for the Analysis of Drugs in Serum Using Capillary Zone Electrophoresis

Deproteinisation with acetonitrile (along with methanol or other reagents) is a useful and rapid technique in analysis of drugs or their metabolites in human serum. In this paper application of this simple technique in biopharmaceutical analysis using capillary electrophoresis (CE) is evaluated. Some drugs with different ionic and protein binding properties were selected and dissolved in human ...